Application

Immunoblotting: Recommended dilution is 1:100

Optimal dilutions must be determined by end-user.

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & InhibitionTumor Markers

Detect Bcl-xL using this Anti-Bcl-xL Antibody, Phospho-specific validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bcl-XL is one of the anti-apoptotic members of the Bcl-2 family of apoptosis regulators and is overexpressed in many human cancers. Studies by Dr. Haldar and his associates suggest that microtubule damaging drugs like Taxol or 2-methoxyestradiol induce phosphorylation of Bcl-XL at serine 62 by kinases such as Jun and this allows those cells to die by apoptosis.

Immunogen

Synthetic phosporylated peptide corresponding to a sequence encompassing serine 62 of human Bcl-xL conjugated to KLH.

Epitope: Phospho-specific

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid rabbit serum containing no preservatives.

Specificity

AB3116 is specific for the Bcl-xL phosphorylated on serine 62. It does not recognize the unphosphorylated form of the protein.

Storage and Stability

Maintain at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.